DNase I (Rnase Free) (2u/ul)
Numera pusi: HC4007B
O le DNase I o se endonuclease e mafai ona eliina le DNA e tasi pe lua.E mafai ona fa'afefeteina fa'amauga phosphodiester e maua ai mono-ma oligodeoxynucleotides o lo'o i ai se vaega 5'-phosphate ma se vaega 3'-OH.Ole maualuga ole galuega ole pH ole DNase I ole 7-8.Ole gaioiga ole DNase I e faʻalagolago ile Ca2+ma e mafai ona faʻagaoioia e ion uʻamea divalent pei ole CO2, Mn2+, Zn2+, ma isi. I luma o Mg2+, DNase E mafai ona ou tipi fa'afuase'i so'o se nofoaga o le DNA fa'alua;A o iai i luma o Mn2+, DNase E mafai ona ou tipiina le DNA fa'alua i le saite lava e tasi, fa'atupu pito tu'u po'o pito pipii ma 1-2 nucleotides fa'aoso.E mafai ona faʻaaogaina mo le faʻaogaina o faʻataʻitaʻiga RNA eseese.
Vaega
| Igoa | 1KU | 5KU |
| Recombinant DNaseI (RNase-sa'oloto) | 500 μL | 5 × 500 μL |
| DNase I Reaction Buffer (10×) | 1 mL | 5 × 1mL |
Tulaga teuina
O lenei oloa e tatau ona teuina i le -25 ~ -15 ℃ mo le 2 tausaga.Fa'amolemole 'alofia le fa'a'aisa fa'aisa.
Faatonuga
Fa'atatau ile aveese ole DNA mai fa'ata'ita'iga RNA mo na'o fa'amatalaga.
1. Fa'amolemole fa'aaoga paipa centrifuge e leai se RNase ma fa'auiga paipa e saunia ai le faiga o tali nei:
| Vaega | Volume (μL) |
| DNase I Reaction Buffer (10×) | 1 |
| Recombinant DNasel (RNase-leai se totogi) | 1 |
| RNA | X |
| RNase-saoloto ddH2O | E oo atu i le 10 |
2. O tulaga faʻaalia e faʻapea: 37 ℃, pe a maeʻa le 15-30 mins, faʻaopoopo se faʻasalaga mulimuli o le 2.5 mM EDTA solution ma faʻafefiloi lelei, ona 65 ℃ mo le 10 min.O le faʻataʻitaʻiga faʻatulagaina e mafai ona faʻaaogaina mo suʻega RT-PCR poʻo RT-qPCR mulimuli, ma isi.
Fa'amatalaga
1. DNase l e maaleale i le denaturation faaletino;A fa'afefiloi, fesuia'i malie le paipa su'ega malulu lelei, aua le lulu malosi.
2. E tatau ona teuina le enzyme i totonu o se pusa aisa poʻo luga o se taele aisa pe a faʻaaogaina, ma e tatau ona teuina i le -20 ℃ i le taimi lava e faʻaaoga ai.
3. O lenei oloa e mo na'o su'esu'ega.
4. Fa'amolemole fa'agaioi i ofu su'esu'e ma totigilima lafoa'i, mo lou saogalemu.
