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2×HiF Taq fa'atasi ma Master Mix HCR2014B Ata Fa'apitoa
  • 2×HiF Taq faaopoopo Master Mix HCR2014B

2×HiF Taq faaopoopo Master Mix


Numera pusi: HCR2014B

afifi: 1ml/5ml/25ml

HIF Taq plus Master Mix (With Dye) o se vaifofo 2×premixed saunia e fa'aoga o lo'o iai le Plus HIF DNA Polymerase, dNTPs, ma fa'apolopolo lelei.

Fa'amatalaga o oloa

Fa'amatalaga o oloa

Numera pusi: HCR2014B

HIF Taq plus Master Mix (With Dye) o se vaifofo 2×premixed saunia e fa'aoga o lo'o iai le Plus HIF DNA Polymerase, dNTPs, ma fa'apolopolo lelei.E lua monoclonal antibodies i le vevela o le potu e taofia ai le gaioiga o le polymerase ma le 3′→5′exonuclease gaioiga e faʻaopoopo i le faʻafefiloi matai mo le faigofie ma sili ona patino Hot Start PCR.O le faʻaopoopoga faʻaopoopo e faʻaopoopoina i le faʻafefiloi matai e tuʻuina atu ai i le enzyme se malosi faʻalautele vaega umi, o le umi o le faʻaopoopoga e mafai ona oʻo atu i le 13 kb, o le enzyme o loʻo i ai le 5′→3′ DNA polymerase activity ma le 3′→5′. gaoioiga exonuclease, o lona faamaoni e 83 taimi o le Taq DNA polymerase, lea e 9 taimi o le DNA polymerase masani.E fetaui lelei mo le faʻalauteleina o faʻataʻitaʻiga lavelave, o le oloa faʻateleina o se pito faʻamaʻi.

2×HIF Taq plus Master Mix(With Dye) o loʻo i ai le lelei o le vave ma le faigofie, maualuga le lagona, malosi faʻapitoa, lelei le mautu, ma isi mea, o le faiga faʻaalia e manaʻomia naʻo le faʻaopoopoina o primers ma templates, ma e mafai ona faʻalauteleina e se lua- fa'asologa o laasaga, fa'afaigofie laasaga fa'ata'ita'i ma fa'asaoina le taimi.O lenei oloa o lo'o i ai ni vaila'au fa'ailoga electrophoresis, ma o oloa PCR e mafai ona fa'aoga sa'o mo electrophoresis.E le gata i lea, o le oloa foi o loʻo i ai se sui puipuia faʻapitoa, ina ia mafai e le faʻafefiloi matai ona faatumauina le gaioiga mautu pe a uma ona toe faʻafefete.


  • Muamua:
  • Sosoo ai:

  • Tulaga Teuteu

    O oloa e tatau ona teuina ile -25~-15℃ mo le 1 tausaga.

     

    Fa'amatalaga

    Fa'amatalaga o oloa

    Master Mix

    Fa'atotonuga

    Amata vevela

    Amata vevela ua fausia

    Fa'atautau

    Tu'u

    Saosaoa tali

    Vave

    Tele (Oloa Mulimuli)

    E oʻo atu i le 13kb

    Tulaga mo felauaiga

    Aisa mago

    Ituaiga oloa

    Premixes PCR maualuga fa'amaoni

     

    Faatonuga

    1.PCR Reaction System

    Vaega

    Volume (μL)

    DNA mamanu

    Talafeagai

    Fa'ailoga muamua (10 μmol/L)

    2.5

    Fa'ata'ita'i (10 μmol/L)

    2.5

    2×HIF Taq faaopoopo Master Mix

    25

    ddH2O

    i le 50

     

    2.Fautuaina le fa'aogaina o mamanu eseese

    Ituaiga mamanu

    Fa'ateleina vaega mai le 1kb i le 10 kb

    Genomic DNA

    50ng-200 ng

    Plasmid poʻo Viral DNA

    10pg-20ng

    cDNA

    1-2.5 µL (Aua le sili atu i le 10% o le tele o tali PCR mulimuli)

     

    3.Polokalama Fa'aliga

    1) Polokalama Laasaga Lua (fa'ata'ita'iga lavelave)

    Laasaga taamilo

    Temp.

    Taimi

    Ta'amilosaga

    Uluai denaturation

    98℃

    3 minu

    1

    Denaturation

    98℃

    10sec

    30-35

    Fa'aopoopoga

    68℃

    30 sec/kb

    Fa'aopoopoga mulimuli

    72 ℃

    5 minu

    1

     

    2) Tolu-Step Protocol (faiga masani)

    Laasaga taamilo

    Temp.

    Taimi

    Ta'amilosaga

    Uluai denaturation

    98℃

    3 minu

    1

    Denaturation

    98℃

    10sec

    30-35

    Fa'asusu

    60℃

    20 sec

    Fa'aopoopoga

    72 ℃

    30 sec/kb

    Fa'aopoopoga mulimuli

    72 ℃

    5 minu

    1

     

    3) Fa'ata'ita'i Fa'ata'ita'i (Fa'ata'ita'iga lavelave)

    Laasaga taamilo

    vevela

    Taimi

    Ta'amilosaga

    Uluai denaturation

    98℃

    3 minu

    1

    Denaturation

    98℃

    10 sec

    15 (1℃ fa'aitiitiga ile ta'amilosaga)

    Fa'alili fa'alelei

    70-55 ℃

    20 sec

    Fa'aopoopoga

    72 ℃

    30 sec/kb

    Denaturation

    98℃

    10 sec

     

    20

    Fa'asusu

    55℃

    20 sec

    Fa'aopoopoga

    72 ℃

    30 sec/kb

    Fa'aopoopoga mulimuli

    72 ℃

    5 minu

    1

     

    Fa'ailoga i lalo o fa'asalalauga fa'aopoopo eseese

    Protocol

    Laasaga Lua

    Laasaga tolu

    Fa'alili fa'alelei

    Fa'amatalaga.

    vave

    feololo

    telegese

    Fa'apitoa

    maualuga

    feololo

    maualuga

    PCR fua

    feololo

    maualuga

    feololo

    Su'e fua

    maualuga

    feololo

    maualuga

     

    Fa'amatalaga

    Fa'amolemole fai le PPE e mana'omia, o se ofu su'esu'e ma totigilima, ia mautinoa lou soifua maloloina ma le saogalemu!

     

    Tusi lau savali iinei ma lafo mai ia i matou