Tele Laasaga Tasi RT-qPCR Pusa Su'esu'e (UDG Plus)
Fa'amatalaga
Multiplex One Step RT-qPCR Kit (UDG Plus) o se pusa su'esu'e RT-qPCR e fa'atatau mo le PCR tasi-plex po'o le tele-plex quantitative PCR fa'aogaina le RNA e fai ma fa'ata'ita'iga (pei o le RNA virus).E fa'aaogaina e fa'alautele ai le DNA fa'alua mai fa'ata'ita'iga RNA fa'atosina e fa'ataga ai se fa'ata'ita'iga vave o fa'atatau RNA.I le fa'asologa o fa'aliliuga fa'asaga i le fa'aliliuga fa'aliliuga e fa'apipi'iina mole mole mole DNA (cDNA) fa'atasi ma le fa'ata'ita'iga RNA.
Fa'agasologa o Tali
Vaega
| Vaega | 200rxns | 1,000rns | 10,000 rxns |
| RNase leai se ddH2O | 3*1ml | 15ml | 150ml |
| 5 * tasi laʻasaga faʻafefiloi | 800μl | 4*1ml | 40ml |
| Tasi la'asaga fa'afefiloi enzyme | 200μl | 1ml | 10ml |
| 50* ROX fa'amatalaga Vai 1 | 80μl | 400μl | 4*1ml |
| 50* ROX fa'amatalaga Vai 2 | 80μl | 400μl | 4*1ml |
a.Tasi-Step Buffer e aofia ai le dNTP Mix ma le Mg2+.
e.O le Enzyme Mix e tele lava ina iai fa'aliliuga fa'aliliu, Hot Start Taq DNA polymerase (suiga o le antibody) ma le inhibitor RNase.
i.Fa'aaogaina e fa'asa'o ai le sese o sinals fluorescene i le va o vaieli eseese.
i.ROX: E te manaʻomia le filifilia o le faʻavasegaina e tusa ai ma le faʻataʻitaʻiga o le meafaigaluega suʻega.
Talosaga
Su'eina o pathogens
su'esu'ega ma su'esu'ega ole tuma
Su'eina o faama'i manu
Su'esu'ega vave o fa'ama'i fa'ama'i
Su'esu'ega o meaola ninii fa'ama'i i totonu o mea'ai
Va'a ma Teuina
Felauaiga:pusa aisa
Tulaga Teuina:Teu i le -30 ~ -15℃.
Soifua Shief:1 tausaga
