Recombinant Deoxyribonuclease I (DNase I, RNase-sa'oloto)
Fa'amatalaga
Recombinant Deoxyribonuclease I, ose endonuclease e mafai ona eliina le DNA tasi pe lua.E fa'asusuina fa'amauga phosphodiester e maua ai mono- ma oligodeoxynucleotides o lo'o iai vaega 5'-phosphate ma vaega 3'-OH.Ole maualuga ole galuega ole pH ole DNase I ole 7-8.O le gaioiga o le DNase I e faʻalagolago i le Ca2 + ma e mafai ona faʻagaoioia e ion uʻamea divalent e pei o Co2 +, Mn2 +, Zn2 +, ma isi. I le i ai o Mg2 +, DNase I mafai ona tipi faʻafuaseʻi le DNA faʻalua i soʻo se nofoaga;a'o iai le Mn2+, DNase I e mafai ona tipi fa'alua le DNA i le saite lava e tasi, fa'atupu pito tu'u po'o 1-2 nuclei pito pipi'i fa'atasi ma nucleotide overhangs.O lenei enzyme e maua mai recombinant E. coli strains, e le o iai le RNase, ma e mafai ona faʻaaogaina mo le gaosiga o faʻataʻitaʻiga RNA eseese.
Fa'asologa o vaila'au
Talosaga
RNA extraction: saunia le RNA e aunoa ma le DNA;
A maeʻa le faʻaliliuga in vitro ma le RNA polymerase, e pei o le T7 RNA Polymerase (Cat #10618), e faʻaaogaina e aveese ai DNA mamanu;
Saunia RNA e leai se DNA i luma o le RT-PCR ma le RT-qPCR;
Fa'aoga fa'atasi ma le DNA Polymerase I (Cat#12903) mo le fa'ailogaina o le DNA i le fa'aliliuga fa'aigoa;
Fa'aaogaina i su'esu'ega tulagavae e fa'avasega ai feso'ota'iga DNA-protein;
Fausia se faletusi vaega fa'afuase'i;
I le su'esu'ega a le TUNEL o le apoptosis, o le DNA genomic vaega na fa'aaogaina e fai ma pulea lelei.
Va'a ma Teuina
Felauaiga:pusa aisa
Tulaga Teuina:Teu i totonu -20℃
Soifua Fa'asao:2 tausaga
Fa'amatalaga:
1. Enzymes e tatau ona teuina i totonu o se pusa aisa poʻo luga o se taele aisa pe a faʻaaogaina, ma e tatau ona teuina i le -20 ° C i le taimi lava e faʻaaoga ai.
2. Mo lou saogalemu ma lou soifua maloloina, fa'amolemole fa'aaoga mea e puipui ai le tagata lava ia (PPE), e pei o ofutino falesuesue ma totigilima fa'aaoga, pe a fa'aoga i lenei oloa.
