DNase I (Rnase Free)(5u/ul)
Pusi Numera: HC4007A
O le DNase I (Deoxyribonuclease I) o se endodeoxyribonuclease e mafai ona eliina le DNA tasi pe lua.Na te iloa ma faʻapipiʻi fusi o le phosphodiester e maua ai monodeoxynucleotides poʻo oligodeoxynucleotides e tasi pe lua faʻatasi ma vaega phosphate i le 5'-terminal ma le hydroxyl i le 3'-terminal.Ole gaioiga ole DNase I e faʻalagolago ile Ca2+ma e mafai ona fa'agaoioia e ion u'amea divalent pei ole Mn2+ma Zn2+.5 mM Ca2+puipuia le enzyme mai le hydrolysis.I luma o Mg2+, e mafai e le enzyme ona iloa faʻafuaseʻi ma faʻapipiʻi soʻo se nofoaga i luga o soʻo se manoa DNA.I luma o Mn2+, e mafai ona iloa i le taimi lava e tasi le manoa e lua o le DNA ma vaeluaina i le toetoe lava o le nofoaga e tasi e fausia ai ni vaega mafolafola DNA pito po o fasi vaega DNA pito pipii ma 1-2 nucleotides protruding.
Vaega
| Igoa | 0.1KU | 1KU | 5 KU | 50 KU |
| DNase I, RNase-saoloto | 20μL | 200μL | 1ml | 10 ml |
| 10 × DNase I Puipui | 1ml | 1ml | 5 × 1mL | 5 × 10 mL |
Tulaga teuina
-25℃~-15℃ mo le teuina;Felauaiga i lalo o pusa aisa.
Faatonuga
1. Saunia le tali tali i le RNase-free tube e tusa ai ma vaega o loʻo lisiina i lalo:
| Vaega | Volume |
| RNA | X µg |
| 10 × DNase I Puipuiga | 1μL |
| DNase I, RNase-sa'oloto(5U/μL) | 1 U ile µg RNA① |
| ddH2O | E oʻo atu i le 10μL |
Fa'aaliga: ①Fa'atatau le voluma ole DNase I e mana'omia ona fa'aopoopo ile aofa'i ole RNA.
2. 37 ℃ mo le 15 minute;
3. Faʻaopoopo le 0.5M EDTA i le faʻasalaga mulimuli o le 2.5mM ~ 5mM, ma vevela i le 65 ℃ mo le 10 minute e taofi ai le tali.E mafai ona fa'aoga sa'o le fa'ata'ita'iga mo le isi tali e pei o le fa'aliliuina fa'aliliufa'ata'ita'iga.
Fa'amatalaga Vaega
E tasi le iunite o loʻo faʻamatalaina o le aofaʻi o le enzyme e faʻaleagaina atoa le 1µg o le pBR322DNA ile 10 minute ile 37℃.
Puleaina lelei
RNase:5U o le DNase I ma le 1.6µg MS2 RNA mo le 4 itula i le 37 ℃ e leai se fa'aleagainafuafuaina e le electrophoresis gel agarose.
Fa'amatalaga
1. Fa'amolemole saunia le 0.5MEDTA e oe.
2. Fa'aaogā le 1U DNase I ile µg ole RNA.Ae peita'i, afai ole RNA e itiiti ifo ile 1µg, fa'amolemole fa'aaoga le 1U DNase I.
3. Faamolemole tuu le enzyme i luga o le aisa i le taimi o le taotoga.
