RT-LAMP Reagent (-20 ℃)
Fa'amatalaga
LAMP o loʻo faʻaaogaina faʻatekonolosi i le taimi nei i le faʻalauteleina o le isothermal.E fa'aogaina le 4-6 fa'amuamua e mafai ona iloa ai 6 vaega fa'apitoa i luga o le kenera fa'atatau, ma fa'alagolago i le malosi o le strand displacement activity a Bst DNA polymerase.E tele auala e iloa ai le LAMP, e aofia ai le auala vali, pH colorimetric method, turbidity method, HNB, calcein, ma isi RT-LAMP o se tasi o ituaiga o LAMP tali faʻatasi ma RNA e fai ma faʻataʻitaʻiga.RT-LAMP Kit (UDG plus) ua atiaʻe e faʻavae i luga o le auala fluorescence ma e iai le Bst DNA Polymerase, thermotolerant reverse transcriptase, murine RNase inhibitor, ma UDGase.O lenei pusa LAMP e maualuga lona lagona, lelei le faʻaogaina muamua, ma sili ona lelei le faʻatinoga i le faʻapitoa, faapalepale, ma le mautu.
Vaega
| 100T | 1000T |
| 600μL | 6ml |
| 100 μL | 1 mL |
1) Bst Buffer e iai le dN(U)TP ma le SYTO-9, ma isi, ma le 1×Bst Buffer ua uma ona iai le 8 mmol/L Mg2+;
2) Bst Enzyme Mix (UDG plus) e iai le UDGase, Reverse Transcriptase, Bst Ⅱ Polymerase, MRI;
Talosaga
LAMP tali
Va'a ma Teuina
Felauaiga:pusa aisa
Tulaga Teuina:Teu i le -20℃
Soifua Shief:6 masina
Lapataiga
Mo lou saogalemu ma lou soifua maloloina, fa'amolemole fai ni ofu talaloa ma totigilima fa'aaoga mo le taotoga.
