Rnase Inhibitor (Glycerol free)
Murine RNase inhibitor o se recombinant murine RNase inhibitor fa'aalia ma fa'amamāina mai E.coli.E fusifusia i le RNase A, B poʻo le C i se fua faatatau 1: 1 e ala i le faʻaogaina o le non-covalent, ma faʻafefe ai le gaioiga o enzymes e tolu ma puipuia le RNA mai le faʻaleagaina.Ae ui i lea, E le aoga i RNase 1, RNase T1, S1 Nuclease, RNase H poʻo RNase mai Aspergillus.Murine RNase inhibitor na tofotofoina e RT-PCR, RT-qPCR ma IVT mRNA, ma sa fetaui ma fefaʻatauaʻiga Reverse transcriptases, DNA polymerases ma RNA polymerases.
Pe a faʻatusatusa i tagata RNase inhibitors, murine RNase inhibitors e le o iai ni cysteine se lua e sili ona maaleale i le faʻamaʻiina e mafua ai le le faʻaaogaina o le inhibitor.O le mea lea e faʻamautu ai i le maualalo o le DTT (itiiti ifo i le 1 mM).O lenei vaega e talafeagai mo le fa'aoga i fa'agaioiga o lo'o i ai le maualuga o le DTT e fa'aleagaina ai le fa'alavelave (fa'ata'ita'iga Real-time RT-PCR).
Atalosaga
O lenei oloa e mafai ona faʻaaogaina lautele i soʻo se faʻataʻitaʻiga e mafai ai e le RNase faʻalavelave e aloese mai le faʻaleagaina o le RNA, e pei o:
1. First-strand cDNA synthesis, RT-PCR, RT-qPCR, ma isi;
2. Puipuia le RNA mai le fa'aleagaina ile in-vitro transcription/fa'aliliuga (eg viral replication in vitro);
3. Inhibition o le RNase gaoioiga i le taimi RNA vavaeeseina ma faamamaina.
Tulaga Teuteu
Teu i le -25～-15℃;
Ta'amilosaga fa'a'aisa-liu ≤ 5 taimi;
Fa'aoga mo le 1 tausaga.
Fa'amatalaga o le iunite
E tasi le iunite o loʻo faʻamatalaina o le aofaʻi o le RNase Inhibitor e manaʻomia e taofia ai le gaioiga o le 5 ng RNase A e 50%.
mamafa mole
RNase Inhibitor (Glycerol-free) o se porotini 50 kDa.
Puleaina lelei
Exonuclease Gaoioiga:
O le incubation o le 40 U o le enzyme ma le 1 μg λ-Hind III digest DNA mo 16 itula i le 37 ° C na mafua ai ona leai se faʻaleagaina o le DNA e pei ona fuafuaina e gel electrophoresis.
Gaoioiga Endonuclease:
O le incubation o le 40 U o le enzyme ma le 1 μg λ DNA mo le 16 itula i le 37 ° C na mafua ai ona leai se faʻaleagaina o le DNA e pei ona fuafuaina e gel electrophoresis.
Nicking Gaoioiga:
O le incubation o le 40 U o le enzyme ma le 1 μg pBR322 mo le 16 itula i le 37 ℃ na iʻu ai i le leai o se faʻaleagaina o le DNA e pei ona fuafuaina e gel electrophoresis.
RNase Gaoioiga:
O le incubation o le 40 U o le enzyme ma le 1.6 μg MS2 RNA mo le 4 itula i le 37 ℃ na iʻu ai i le leai o se faʻaleagaina o le RNA e pei ona fuafuaina e gel electrophoresis.
E.coli DNA:
40 U o le Enzyme e iloa e TaqMan qPCR.Ole DNA E.coli ole ≤ 0. 1pg/40U.
Notes
1. Aua le luluina pe faʻaoso faʻamalosi e puipuia ai le enzyme inactivation.
2. RNase inhibitor o loʻo galue i le vevela e amata mai i le 25 ℃ i le 55 ℃ ma e le faʻaaogaina ile ≥65 ℃.
3. O le gaioiga a RNase H, RNase 1, ma RNase T1 e le faʻasaina.
4.O le pH mo le faʻalavelaveina o le gaioiga o le RNase e lautele (gaoioiga i le pH 5-9), faʻaalia le maualuga o le gaioiga ile pH 7-8.
