dATP/ dCTP/ dGTP/ dTTP/ dUTP (100mM )
Fa'amatalaga
O lenei oloa e leai se lanu vaifofo vai.E fetaui mo su'esu'ega fa'ata'ita'iga fa'aola mole mole masani e pei o le fa'alauteleina o le PCR, PCR taimi moni, cDNA po'o le fa'asologa o DNA masani, fa'asologa o DNA ma fa'ailoga.E mafai ona faʻafefeteina i le vai ultra-mama, ma fetuutuunai i le pH 7.0 faʻatasi ai ma le maualuga o le mama NaOH solution, ma le mama ≥ 99% (HPLC).A maeʻa ona iloa, e le o iai le DNase, RNase ma le phosphotase.E mafai ona fa'aoga sa'o i le tele o fa'agaioiga mole mole masani e pei ole PCR.
Fa'asologa o vaila'au
Fa'amatalaga
| Su'ega mea | Fa'amatalaga |
| Fa'aaliga | Fofo manino le lanu |
| Fa'atotonuga | 100mM ±3% |
| Mama (HPLC) | ≥99% |
| PH(22-25°C) | 7.0±0.1 |
| Pulea lelei1.O lenei sauniuniga e leai se afaina o le DNase ma RNase 2. O lenei tele o le dNTP ua fa'ata'ita'iina fa'atinoga ile Taq DNA polymerase ma le Pfu DNA polymerase. Ole tele ole NTP ua fa'ata'ita'iina ile Q-PCR | |
Talosaga
PCR mamao (20 kb)
fa'asologa o le cDNA ma le RT-PCR
PCR taimi moni
PCR masani
PCR maualuga fa'amaoni
Va'a ma Teuina
Felauaiga:pusa aisa
Tulaga Teuina:Teu i le -25～-15℃
Fautuaina aso toe su'ega:2 tausaga
Fa'amatalaga
1) E mafai ona solo i le vevela o le potu.A maeʻa ona faʻamavae, e tatau ona teuina i totonu o se pusa aisa poʻo le taele aisa.A maeʻa ona faʻaoga, e tatau ona teuina ile -25～-15 ℃ vave.
2) Mo lou saogalemu ma lou soifua maloloina, fa'amolemole fa'aofu i ofu su'esu'e ma totigilima fa'aaoga mo le fa'agaioiga.
