Poly A Carrier RNA
Numera pusi: HC4001A
Poly A, polyadenylate, o se faʻafefiloi o 100 ~ 10000 polyadenylates, lea e polymerized e polynucleotide phosphorylase in vitro.I vivo, poly (a) faʻaopoopo i le 3-terminal o le mRNA e ala ile enzyme e faʻaleleia ai le mautu o le mRNA.I le faʻaaogaina o le suʻeina o le nucleic acid, faʻaopoopoina le poly A i le lysate poʻo le faʻapipiʻiina o vaifofo e mafai ona faʻaleleia ai le gaosiga o le DNA ma le RNA.Le masiniole poly A e fa'aleleia ai le gaosiga ole nucleic acid e fa'apea:
1. Fa'afeso'ota'i fa'ama'a'a ma le fa'asa'o i luga o mea.O le tele o mea polypropylene o lo'o i ai le eletise fa'amau i luga, lea o le a fa'afefete ai nucleic acids.Carrier RNA e mafai ona fa'atumuina nei meaa'afiaga ole adsorption ma fa'aitiitia ai le leiloa ole nucleic acids.
2. Inactivate trace nucleases: E iai nucleases eseese i meaola faʻataʻitaʻiga masiosiomaga.O le Poly A e mafai ona fa'agataina fa'amauina nucleases i le fa'asaoina po'o le fa'asaoina o la'asaga ifa'aleleia le fa'atupuina ma le mautu o su'esu'ega nucleic.
3. Coprecipitation: I le fa'amamāina o le acid nucleic step o le ava malosi fa'afefeteina po'o le fusifusia, poly A e mafai ona fa'atasi ma le fa'atatau nucleic acid po'o le faia o ni vaega polymer efaaleleia le toe faaleleia.
Tulaga teuina
-20 ~ 8 ℃, teuina mago, teuina umi umi e tatau ona tuʻu i -20 ℃
Fa'amatalaga
| Numera CAS | 26763-19-9 |
| Fa'aaliga | Pa'u pa'epa'e lyophilized |
| Mama | 99% |
| Molecular Weight | 700-3500 KDa |
Fa'aoga auala
Ave se aofa'i talafeagai o le pauta lyophilized, fa'aopoopo i ai le vai fa'afefeteina DEPC po'o le vaifofo masima guanidinefa'amavae i le 0.1-1ug/uL, ona fa'aputu lea ma teu i le -20°C.
Talosaga
1.Virus DNA / RNA extraction: faʻaopoopo le 1-5ug Carrier RNA i le lysate e mafai ona faʻaleleia le gaosiga o le RNA / DNA, faʻamautu le faʻaogaina o le nucleic acid ma aloese mai le faʻaleagaina o le nucleic acid mama i le taimi o le teuina.
2. I le micro DNA / RNA faʻapipiʻiina e ala i le koluma membrane method (<1ug), faʻaopoopo le carrier RNA i le 1-5ug e faʻaleleia e faʻaleleia ai le gaosiga o le nucleic acid.
3. I le ava malosi fa'asalalau nucleic acid precipitation ma le fa'atonuga laasaga, o le fa'aopoopoga o le 1-2ug carrier RNA e fesoasoani e fa'aleleia atili ai le toe fa'aleleia o le vaega pu'upu'u RNA.
4. I le quantitative probe PCR reaction solution, faʻaopoopo 10-100ng carrier RNA i le tali tali e fesoasoani e faʻaleleia ai le lagona ma faʻaitiitia le C.Ttaua.
