Tasi Laasaga RT-qPCR pusa su'esu'e
Fa'amatalaga
Tasi Laasaga qRT-PCR Su'esu'ega Kit ua fa'apitoa mo le qPCR e fa'aoga sa'o le RNA (fa'ata'ita'iga virus RNA) e fai ma fa'ata'ita'iga.I le fa'aaogaina o gene specific primers (GSP), e mafai ona fa'amae'a le fa'aliliuga fa'aliliu ma le qPCR i totonu o le fa'agaau e tasi, e fa'aitiitia ai faiga fa'apa paipa ma le lamatiaga o le fa'aleagaina.E mafai ona le faʻaaogaina i le 55 ℃ e aunoa ma le aʻafiaina o le lelei ma le maaleale o le qRT-PCR.Tasi Laasaga qRT-PCR Su'esu'e Kit o lo'o tu'uina atu ile Master Mix.O le 5 × One Step Mix o loʻo i ai se paʻu sili ona lelei ma le dNTP/dUTP Mix, ma e talafeagai mo faiga faʻapitoa e suʻesuʻe ai e faʻavae i luga ole suʻega faʻaigoaina o le fluorescence (eg TaqMan).
Mataupu Faavae Autu o le RT-qPCR
Fa'amatalaga
| Mea Su'ega | Fa'amatalaga | I'uga |
| (SDS PAGE) Mama o le Enzyme Stock(SDS PAGE) | ≥95% | Pasia |
| Gaoioiga Endonuclease | Le iloa | Pasia |
| Gaoioiga Exodulease | Le iloa | Pasia |
| Gaoioiga Rnase | Le iloa | Pasia |
| E.coli DNA totoe | <1 kopi/60 | Pasia |
| Fuafuaga-Assay System | 90%≤110% | Pasia |
Vaega
| Vaega | 100rxns | 1,000rns | 5,000 rxns |
| RNase leai se ddH2O | 2*1ml | 20ml | 100ml |
| 5 * tasi laʻasaga faʻafefiloi | 600μl | 6*1ml | 30ml |
| Tasi la'asaga fa'afefiloi enzyme | 150μl | 2*750μl | 7.5ml |
| 50* ROX fa'amatalaga Vai 1 | 60μl | 600μl | 3*1ml |
| 50* ROX fa'amatalaga Vai 2 | 60μl | 600μl | 3*1ml |
a.Tasi-Step Buffer e aofia ai le dNTP Mix ma le Mg2+.
e.O le Enzyme Mix e tele lava ina iai le fa'afeagai
transcriptase, Hot Start Taq DNA polymerase (suiga antibody) ma le inhibitor RNase.
i.Fa'aaogaina e fa'asa'o ai le sese o sinals fluorescene i le va o vaieli eseese.
o.ROX: E te manaʻomia le filifilia o le faʻavasegaina e tusa ai ma le faʻataʻitaʻiga o le meafaigaluega suʻega.
Talosaga
Su'esu'ega QPCR
Va'a ma Teuina
Va'a:pusa aisa
Tulaga Teuina:Teu i le -20℃.
Soifua Shief:18 masina
