Ultra Nuclease
UltraNuclease o se genetically engineeredendonuclaese e maua mai Serratia marcescens, lea e mafai ona faʻaleagaina le DNA poʻo le RNA, pe faʻalua pe tasi, laina laina poʻo le lapotopoto i lalo o le tele o tulaga, faʻaleagaina atoa le nucleic acids i le 5'-monophosphate oligonucleotides ma le umi 3-5base. .Ina ua maeʻa suiga faʻainisinia, sa faʻafefeteina le oloa, faʻaalia, ma faʻamamāina i le Escherichia coli (E. coli), lea e faʻaitiitia ai le viscosity o le supernatant cell ma le cell lysate suʻesuʻega faʻasaienisi, ae faʻaleleia atili le faʻamamaina lelei ma suʻesuʻega faʻatino o porotini.E mafai foʻi ona faʻaaogaina i le faʻamaʻi faʻamaʻi, faʻamamāina o siama, gaosiga o tui, palatini ma polysaccharide alamanuia vailaʻau e fai ma faʻamaʻi faʻamaʻi faʻamaʻi nucleic acid reagent.
Fa'ailoga oloa
| CAS Nu. | 9025-65-4 |
| EC Nu. | |
| Molecular Weight | 30kDa |
| Isoelectric Point | 6.85 |
| Polotini Mama | ≥99%（ SDS-IUUU MA SEC-HPLC） |
| Gaoioiga Fa'apitoa | ≥1.1×106U/mg |
| Temperature aupito maualuga | 37°C |
| pH sili ona lelei | 8.0 |
| Gaoioiga Porotease | leaga |
| Avega olaola | <10CFU/100,000U |
| O totoe Host-cell Protein | ≤10ppm |
| Uamea Mamafa | ≤10ppm |
| Endotoxin siama | <0.25EU/1000U |
| Taofi Taofi | 20mM Tris-HCl, pH 8.0, 2mM MgCl2 ， 20mM NaCl, 50% Glycerol |
Tulaga teuina
≤0°C felauaiga;-25~-15°C Teuina, 2 tausaga le aoga (aloese mai le malulu-liu).
Fa'amatalaga Vaega
Ole aofaʻi ole enzyme na faʻaaogaina e suia ai le aoga ole absorption ole △A260 ile 1.0 i totonu ole 30min ile 37 °C, pH 8.0, e tutusa ma le 37μg salmon sperm DNA digested e ala i le tipiina i oligonucleotides, na faʻamatalaina o se iunite galue (U).
Puleaina lelei
O totoe Host-cell Protein: ELISA pusa
•Protease Toega: 250KU/mL UltraNuclease tali atu ma substrate mo 60min, leai se gaoioiga na iloa.
•Endotoxin siama: LAL-Su'ega, Pharmacopoeia of the People's Republic of China Volume 4 (2020 Edition) gel limitation method.Tulafono Lautele (1143).
•avega olaola: Pharmacopoeia of the People's Republic of China Volume 4 (2020 Edition)— General
Tulafono mo Su'ega Fa'amama (1101), PRC National Standard, GB 4789.2-2016.
•Uamea Mamafa:ICP-AES, HJ776-2015.
Fa'agaioiga
O le gaioiga o le UltraNuclease na matua faʻalavelaveina pe a sili atu le maualuga o le SDS i le 0.1% poʻo le EDTA
fa'atonuga na sili atu i le 1mM. Surfactant Triton X- 100, Tween 20 ma Tween 80 sa leai se aafiaga i le nuclease
meatotino pe a o'o i lalo ole 1.5%.
| Fa'agaioiga | Fa'atonuga lelei | Fa'agaioiga aoga |
| vevela | 37℃ | 0-45 ℃ |
| pH | 8.0-9.2 | 6.0- 11.0 |
| Mg2+ | 1-2mM | 1- 15mM |
| DTT | 0- 100mM | >100mM |
| 2-Mercaptoethanol | 0- 100mM | >100mM |
| Ion uamea monovalent (Na+, K+ ma isi) | 0-20mM | 0-200mM |
| PO43- | 0- 10mM | 0- 100mM |
Fa'aoga ma Fuafuaga
• Ave'ese mai fafo ole nucleic acid mai oloa tui, fa'aitiitia le lamatiaga ole fa'a'ona ole nucleic acid ma fa'aleleia atili le saogalemu ole oloa.
• Fa'aitiitia le viscosity o le sua fafaga e mafua mai i le nucleic acid, fa'apu'upu'u taimi fa'agaioiga ma fa'ateleina le maua mai o polotini.
• Aveese le nucleic acid o lo'o afifi ai vaega (virus, inclusion body, etc.), lea e fa'alelei
i le tatalaina ma le faamamaina o le vaega.
| Ituaiga Fa'ata'ita'i | Gaosi Polotini | Virus, Tu'i | Vailaau Vailaau |
| Numera sela | 1g le mamafa susu (toe tu'u ma le 10ml pa'u) | 1L fa'afefete supa vai | 1L aganuu |
| Tulaga Laiti | 250U | 100U | 100U |
| Fusi Fautuaina | 2500U | 25000U | 5000U |
• O togafitiga Nuclease e mafai ona fa'aleleia atili ai le fofo ma le toe fa'aleleia o le fa'ata'ita'iga mo koluma chromatography, electrophoresis ma su'esu'ega blotting.
• I le togafitiga o kene, e aveese le nucleic acid ina ia maua ai siama e feso'ota'i adeno mama.
