BspQI Restriction Endonuclease
Fa'amatalaga
BspQI e mafai ona toe faʻaalia i le E. coli e mafai ona iloa nofoaga faʻapitoa ma gaosia i lalo o tulaga GMP.E faʻaaogaina lautele i faʻafanua faʻafanua, cloning ma isi fanua.O lenei enzyme e vave tipiina le DNA mo le faʻaogaina lelei o gene.
5'······GGTCTC(N)1·····3'
3'·····CCAGAG(N)4·····5'
Fa'atonuga vaila'au
Fa'amatalaga Vaega
E tasi le iunite o lo'o fa'amatalaina o le aofa'i o le enzyme e mana'omia e fa'alilo ai le 1 µg o le λ DNA i le 1 itula i le 50°C i le aofa'i o le tali e 50 µl.
Oloa Fa'ailoga
• O le fa'atapula'a endonuclease e maualuga le ga'o fa'ama'i ma fa'amae'a vave ai le ga'o.
• Fa'aitiitia le fa'aogaina ole endonuclease e fa'amautinoa ai le tipiina o le "scalpel".
• Leai se faiga BSA, fa'aitiitia ai le fa'aleagaina o le vevela.
Fa'amatalaga
| Mea Su'ega | Fa'amatalaga |
| Polotini Mama | ＞98% |
| Protease toega | Leai se mea na iloa |
| So'oga ma va'aiga tipi | Fa'aliga lelei> 95% |
| DNase | Leai se mea na iloa |
| Nicaknase | Leai se mea na iloa |
Felauaiga ma le teuina
Felauaiga:La'uina i lalo ole 0 °C
Teuina:Teu i le -20 °C
Fautuaina le toe su'ega Olaga:2 tausaga
