Ultra Nuclease
Fa'amatalaga
UltraNuclease o se endonuclaese faʻainisinia faʻainisinia e maua mai i le Serratia marcescens, lea e mafai ona faʻaleagaina le DNA poʻo le RNA, pe faʻalua pe tasi, laina laina poʻo le lapotopoto i lalo o le tele o tulaga, faʻaleagaina atoatoa nucleic acids i 5'-monophosphate oligonucleotides ma 3-5 umi faavae.
Ina ua maeʻa suiga faʻainisinia kenera, na faʻafefeteina le oloa, faʻaalia, ma faʻamamāina i Escherichia coli (E. coli), lea e faʻaitiitia ai le viscosity o le supernatant cell ma le cell lysate i suʻesuʻega faasaienisi, ae faʻaleleia atili le faʻamamaina lelei ma suʻesuʻega galue o le porotini.E mafai foʻi ona faʻaaogaina i le faʻamaʻi faʻamaʻi, faʻamamaina o siama, gaosiga o tui, polotini ma le polysaccharide pharmaceutical alamanuia e avea o se talimalo toega nucleic acid reagent.
Fa'atonuga vaila'au
Fa'amatalaga Vaega
Ole aofaʻi ole enzyme na faʻaaogaina e suia ai le aoga ole absorption ole △A260 ile 1.0 i totonu ole 30min ile 37 °C, pH 8.0, e tutusa ma le 37μg salmon sperm DNA digested e ala i le tipiina i oligonucleotides, na faʻamatalaina o se vaega malosi.
Fa'aoga ma Fuafuaga
• Ave'ese mai fafo ole nucleic acid mai oloa tui, fa'aitiitia le lamatiaga ole fa'a'ona ole nucleic acid ma fa'aleleia atili le saogalemu ole oloa.
• Fa'aitiitia le viscosity o le sua fafaga e mafua mai i le nucleic acid, fa'apu'upu'u taimi fa'agaioiga ma fa'ateleina le maua mai o polotini.
• Aveese le nucleic acid o lo'o afifi ai vaega (virus, inclusion body, etc.), lea e fa'amalieina ai le fa'asa'olotoina ma le fa'amamaina o le vaega.
• O togafitiga Nuclease e mafai ona fa'aleleia atili ai le fofo ma le toe fa'aleleia o le fa'ata'ita'iga mo koluma chromatography, electrophoresis ma su'esu'ega blotting.
• I le togafitiga o kenera, e aveese le nucleic acid ina ia maua ai siama e feso'ota'i adeno mama.
Fa'amatalaga
| Mea Su'ega | Fa'amatalaga |
| Fa'amatalaga | E manino ma le lanu |
| Gaoioiga | ≥ 250 U/ul |
| Gaoioiga faapitoa | ≥1.1*106U/mg |
| Mama (SDS-PAGE) | ≥ 99.0% |
| Protease | Leai se mea na iloa |
| avega olaola | ＜10 cfu/100,000U |
| Endotoxine(su'ega LAL) | ＜ 0.25EU/1,000U |
Felauaiga ma le teuina
Felauaiga:La'uina i lalo ole 0 °C
Teuina:Teu i le -25~-15°C
Fautuaina le toe su'ega Olaga:2 tausaga (aloese mai le fa'aisa-liliu)
