BsaI Restriction Endonuclease

Mama: 20U/μL

afifi: 50μL, 250μL, 1ml,10ml, 100ml

Fa'amatalaga Oloa

Fa'amatalaga

O le BsaI restriction enzyme o se ituaiga II restriction endonuclease e maua mai i le polotini recombinant ua fa'ailogaina e le gene BspQI i Bacillus sphaericus fa'aalia e E.coli.Fa'atapula'a endonucleases o lo'o fa'aaogaina lautele i fa'afanua o kenera, fa'a-cloning ma isi matā'upu.O lenei enzyme e vave tipiina le DNA mo le faʻaogaina lelei ole gene Bsa I, o le IIs restriction endonuclease restriction endonuclease, e maua mai i le recombinant E. coli strain o loʻo tauaveina le gene BsaI faʻapipiʻi ma suia mai le Bacillus stearothermophilus.o lo'o i ai le tulaga fa'apitoa e pei o enzymes fa'ale-aganu'u ma fa'aitiitia le gaioiga o fetu.O lona fa'asologa fa'ailoga ma nofoaga va'aiga e fa'apea:

5'·····GGTCTC(N)······ ··3'

3'······CCAGAG(NNNNNN)······5'

Fa'atonuga vaila'au

Fa'ailoga oloa

• O le endonuclease fa'atapula'a e maualuga le gaioioiga fa'ama'i ma fa'amae'a fa'ato'a fa'atosina vave.

• Fa'aitiitia le fa'aogaina ole endonuclease e fa'amautinoa ai le tipiina o le "scalpel".

• Leai se faiga BSA, fa'aitiitia ai le fa'aleagaina o le vevela.

Fa'amatalaga

Mea Su'ega	Fa'amatalaga
Gaoioiga Enzyme	20 U/µL
Mama (SDS-PAGE)	≥95%
Rnase	Leai se mea na iloa
Gaoioiga DNase Le'o Fa'apitoa	Le iloa
Fetu Gaoioiga	Le iloa
Ligation ma Recutting	A maeʻa le faʻamaʻiina, e mafai ona faʻapipiʻi vaega o DNA.Ma e mafai ona toe tipiina nei fasi vaega i le Bsal.
Endotoxins(su'ega LAL)	≤10 EU/mg
E.Coli DNA	≤1 Kopi/2U

Pulea lelei

Mama ≥ 95%, Leai se DNase, RNase gaoioiga, Host DNA toega ≤ 100pg/mg, fa'ato'aga polotini talimalo ≤ -50ppm, endotoxin toega ≤ 10EU/mg, Leai se protease gaoioiga, sterile, saoloto mai mycoplasma

Fa'atatauga

Eli le plasmid e saunia ai se fasi vaega DNA laina i le pito o le Poly (A/T/C/G);

Linearize plasmid template a'o le'i tusia i totonu o le vitro;

Fa'atapula'a fa'aliga;

Fa'amatalaga Vaega

E tasi le iunite o loʻo faʻamatalaina o le aofaʻi o le enzyme e manaʻomia e faʻafefe ai le 1 µg o le Internal Control DNA i le 1 itula i le 37°C i le aofaʻi o le tali o le 50 μL.