RT-LAMP Fluorescent (Lyophilized polo)
O le RT-LAMP Fluorescent Master Mix (Lyophilized Powder) o lo'o i ai tali fa'afefe, Bst DNA polymerase ma le vevela fa'asaga i tua transcriptase, lyophilized puipuiga, ma le lanu felanulanua'i.O le oloa o loʻo i ai i foliga o lyophilized beads, ma e naʻo primers ma faʻataʻitaʻiga e manaʻomia ona faʻaopoopo mo le faʻaaogaina.
Vaega Reagent
RT-LAMP Fluorescent Master Mix (Lyophilized Powder)
Fa'aoga
Mo DNA poʻo RNA isothermal amplification
Tulaga Teuteu
Felauaiga ma teuina ile 2~8°C.
Ola fa'asa: 12 masina.
Polokalama
1. Ave ese le numera tutusa o lopa lyophilized e tusa ai ma le numera o suʻega.
2. Sauniuniga faiga tali
| Vaega | Fuafuaga |
| RT-LAMP Fluorescent Master Mix (Lyophilized Powder) | 2 pulu |
| vali felanulanua'i | x μL |
| 10 × Fa'afefiloi Muamua a | 5 μL |
| Fa'ata'ita'iga DNA/RNA b | n μL |
| Aofa'iga | 50 μL |
Fa'amatalaga:
1) a.10×Primer Mix : 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B;
2) e.Ole fa'ata'ita'iga ole nucleic acid e fautuaina e fa'amavae ile vai DEPC.
Hyasen Biotechnology Co., Ltd
3) Polokalama tali
| vevela | Taimi | Ta'amilosaga |
| 65℃ | 60s a | 30 |
Fa'amatalaga:
1. Aoina fa'ailoga felanulanua'i
Fa'amatalaga
1. O le vevela o le tali e mafai ona faʻamalieina i le va o le 62 ° C ma le 68 ° C e tusa ai ma le mea muamuatulaga;
2. O le faʻataʻitaʻiga e tatau ona faʻataʻitaʻiina le gaioiga, e aofia ai le saunia o le faiga o tali,fa'ata'ita'iga fa'ata'ita'iga ma le tu'uina;
3. Ina ia aloese mai le faaleagaina, e fautuaina e saunia le faiga o tali i se laulau ultra-mamama fa'aopoopo ni fa'ata'ita'iga i le fa'aulu asu i isi potu e 'alofia ai fa'alavelave fa'aletonu sese.
