Fosfatase Alkaline (ALP)
Fa'amatalaga
O le Alkaline Phosphatase e maua mai i le toe faʻafouina o le E. coli strain o loʻo tauaveina le gene TAB5.O le enzyme e fa'atupuina le dephosphorylation o le 5' ma le 3' pito o DNA ma RNA phosphomonoesters.E le gata i lea, e faʻafefeteina ribose, faʻapea foʻi ma deoxyribonucleoside triphosphates (NTPs ma dNTPs).TAB5 Alkaline Phosphatase e galue i le 5' pito fa'aoso, 5' pito pito ma'a.E mafai ona fa'aogaina le Phosphatase i le tele o talosaga mole mole biology, e pei o le fa'ailoina po'o le fa'ailoga fa'ai'uga e aveese ai pito phosphorylated o le DNA po'o le RNA.I suʻesuʻega faʻapipiʻi, o le dephosphorylation e taofia ai le DNA plasmid laina mai le faʻaogaina o le tagata lava ia.E mafai fo'i ona fa'aleagaina dNTPs e le'i tu'ufa'atasia i tali PCR e saunia ai se fa'ata'ita'iga mo le fa'asologa o DNA.O le enzyme e atoatoa ma e le mafai ona toe faʻaaogaina e ala i le faʻamafanafanaina i le 70 ° C mo le 5 minute, ma faʻapena le aveesea o le phosphatase aʻo leʻi faʻaogaina pe faʻamutaina le faʻailogaina e le manaʻomia.
Fa'aoga
1. Alkaline phosphatase faʻatasi ma polotini (antibodies, streptavidin ma isi,) e mafai ona faʻamaonia faapitoa mole mole, ma e mafai ona faʻaaogaina i le ELISA, WB ma le suʻesuʻeina o le histochemical;
2. Alkaline phosphatase e mafai ona faʻaaogaina e faʻaumatia ai le 5 '-terminal o DNA poʻo RNA e puipuia ai le soʻotaga o ia lava;
3. O le DNA dephosphorylated o loʻo i luga poʻo le RNA e mafai ona faʻailogaina e ala i le leitio-faʻailoga phosphates (e ala i le T4 poly-nucleotide kinase)
Fa'atonuga vaila'au
Fa'amatalaga
| Su'ega Mea | Fa'amatalaga |
| Gaoioiga Enzyme | 5U/μL |
| Gaoioiga Endonuclease | Le iloa |
| Gaoioiga Exonuuclease | Le iloa |
| Gaoioiga Nicking | Le iloa |
| RNase Gaoioiga | Le iloa |
| E.coli DNA | ≤1kopi/5U |
| Endotoxin | LAL-Su'ega, ≤10EU/mg |
| Mama | ≥95% |
Felauaiga ma le teuina
Felauaiga:Ambient
Teuina:Teu i le 2-8°C
Fautuaina le toe su'egaSoifua:2 tausaga
