D-Lactate dehydrogenase(LDH)
Fa'amatalaga
O le enzyme e faʻaaogaina mo le aveeseina o le pyruvate i faʻamoemoega o loʻo galue ma le NADH (triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenase).
Fa'aoga
1. Alkaline phosphatase faʻapipiʻi i polotini (antibody, streptavidin etc,) e mafai ona faʻamaonia faapitoa mole mole, ma e mafai ona faʻaaogaina i le ELISA, WB ma le suʻesuʻeina o le histochemical;
2. Alkaline phosphatase e mafai ona faʻaaogaina e faʻaumatia ai le 5 '-terminal o DNA poʻo RNA e puipuia ai le soʻotaga o ia lava;
3. O le DNA dephosphorylated o loʻo i luga poʻo le RNA e mafai ona faʻailogaina e ala i le leitio-faʻailoga phosphates (e ala i le T4 poly-nucleotide kinase)
Fa'atonuga vaila'au
Faiga fa'afoliga
D-Lactate + NAD+→ Pyruvate +NADH + H+
Fa'amatalaga
| Mea Su'ega | Fa'amatalaga |
| Fa'amatalaga | Pa'u pa'epa'e amorphous, lyophilized |
| Gaoioiga | ≥200U/mg |
| Mama (SDS-PAGE) | ≥90% |
| Soluble(10mg pauta/mL) | manino |
| NADH/NADPH oxidase | ≤0.01% |
| Malate dehydrogenase | ≤0.005% |
| Glutamate dehydrogenase | ≤0.003% |
| Pyruvate kinase | ≤0.03% |
| Glutamate Dehydrogenase | ≤0.003% |
| Aspartate aminotransferase | ≤0.001% |
| Alaminina aminotransferase | ≤0.001% |
Felauaiga ma le teuina
Felauaiga:Ambient
Teuina:Teu i le -20°C(Taimi umi), 2-8°C (pu'upu'u)
Fautuaina le toe su'egaSoifua:2 tausaga
