M-MLV Reverse Transcriptase (Glycerol free)
O se lyophilizable Reverse Transcriptase.E mafai ona faʻaaogaina i lalo ole lyophilization tekonolosi aʻo faʻatumauina le tele o faʻasologa faʻaliliuga faʻasolosolo ma mautu.O lenei oloa e le o iai ni mea fa'apitoa, fa'amolemole fa'aopoopo lau oe lava pe'a mana'omia.
Vaega
| Vaega | HC2005A-01 (10,000U) | HC2005A-02 (40,000U) |
| Reverse Transcriptase (Glycerol Free) (200U/μL) | 50 μL | 200 μL |
| 5 × Taofi | 200 μL | 800 μL |
Talosaga:
E fa'atatau mo tali e tasi le la'asaga RT-qPCR.
Tulaga Teuteu
Teu i le -30 ~ -15°C ma felauaiga ile ≤0°C.
Fa'amatalaga Vaega
E tasi le iunite (U) o loʻo faʻamatalaina o le aofaʻi o le enzyme e faʻapipiʻi ai le 1 nmol o le dTTP i mea e le mafai ona faʻaaogaina i le 10mins i le 37 ° C, ma le Poly(rA)·Oligo (dT) e fai ma faʻataʻitaʻiga / primer.
Fa'amatalaga
Mo na'o su'esu'ega fa'aoga.E le mo le faʻaaogaina i faʻataʻitaʻiga faʻamaonia.
1.Fa'amolemole fa'amama le nofoaga fa'ata'ita'i;Fai totigilima ma matapulepule;Fa'aaogā mea fa'aaogā e leai se RNase e pei o paipa fa'acentrifuge ma pito paipa.
2.Taofi le RNA i luga o le aisa e aloese ai mai le faʻaleagaina.
3.O faʻataʻitaʻiga RNA maualuga e fautuaina e ausia ai le maualuga o le lelei o le faʻaliliuina o tusitusiga.
