Hexokinase (HK)
Fa'amatalaga
Fa'aaogā le Hexokinase mo le su'eina o le D-glucose, D-fructose, ma le D-sorbitol i mea'ai po'o fa'ata'ita'iga su'esu'ega.O le enzyme e faʻaaogaina foi mo le suʻeina o isi saccharides e mafai ona liua i le kulukose poʻo le fructose, ma o lea e aoga i le suʻega o le tele o glycosides.
Afai o le Hexokinase e faʻaaogaina faʻatasi ma le glucose-6-phosphate dehydrogenase (G6P-DH) * (suʻesuʻeina le glucose6-phosphate na faia e Hexokinase), e le tatau ona maualuga le maualuga o le phosphate faʻataʻitaʻiga ona o le G6P-DH e faʻatauvaʻa faʻasaina e le phosphate.
Fa'atonuga vaila'au
Mataupu Faavae o Tali
D-Hexose + ATP --Mg2+→ D-Hexose-6-phosphate + ADP
Fa'amatalaga
| Su'ega Mea | Fa'amatalaga |
| Fa'amatalaga | Pa'epa'e i sina samasama pauta amorphous, lyophilized |
| Gaoioiga | ≥30U/mg |
| Mama (SDS-PAGE) | ≥90% |
| Solubility(10mg pauta/ml) | manino |
| Protease | ≤0.01% |
| ATPase | ≤0.03% |
| Phosphoglucose isomerase | ≤0.001% |
| Creatine phosphokinase | ≤0.001% |
| Glucose-6-Phosphate dehydrogenase | ≤0.01% |
| NADH/NADPH oxidase | ≤0.01% |
Felauaiga ma le teuina
Felauaiga: Ambient
Teuina:Teu i le -20°C(Taimi umi), 2-8°C (pu'upu'u)
Fautuaina le toe su'egaSoifua:1 tausaga
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