Kulukose Dehydrogenase(GDH)
Fa'amatalaga
Glutamate Dehydrogenase (GDH) o se enzyme mitochondrial lea e faʻamalosia ai le toe faʻafoʻiina o le oxidative deamination o le glutamate i le a-ketoglutarate ma avea o se fesoʻotaʻiga autu i le va o auala anabolic ma catabolic.I mammals, GDH e noatia i tulafono faatonutonu allosteric ma e maualuga le gaioiga i le ate, fatugaʻo, faiʻai, ma le pancreas.O le gaioiga o le GDH i totonu o le toto e mafai ona faʻaaogaina e vaʻavaʻai ai i le va o faʻamaʻi ate ona o le mumu o le ate, lea e le faʻaalia ai le maualuga o le GDH serum, ma faʻamaʻi e mafua ai le necrosis hepatocyte, lea e maua ai le GDH maualuga.
Ole gaioiga ole GDH e fuafua ile su'esu'ega ole enzyme lea e fa'aaogaina ai le glutamate e le GDH e fa'atupuina le NADH, lea e tali atu ma se su'esu'ega e fa'atupu ai se mea lanu (450 nm) fa'atusatusa i le gaioiga GDH o lo'o iai.E tasi le iunite o le GDH o le aofaʻi o enzyme e maua ai le 1.0 mmole o le NADH ile minute ile pH 7.6 ile 37 °C
Fa'atonuga vaila'au
Faiga fa'afoliga
D-Glucose + taliaina → D-Glucono-1,5-lactone + fa'aitiitia le taliaina
Fa'amatalaga
| Mea Su'ega | Fa'amatalaga |
| Fa'amatalaga | Pa'u pa'epa'e amorphous, lyophilized |
| Gaoioiga | ≥160U/mg |
| Mama (SDS-PAGE) | ≥90% |
| Solubility(10 mg pauta/mL) | manino |
| Fa'aleaga enzymes | |
| Glucose dehydrogenase (NAD) | ≤0.02% |
| Hexokinase | ≤0.02% |
| A-Glucosidase | ≤0.02% |
Felauaiga ma le teuina
Felauaiga: pusa aisa
Teuina:Teu i le -25~-15°C(Taimi umi), 2-8°C (pu'upu'u)
Fautuaina le toe su'egaSoifua: 18 masina
