Fructosyl-peptide Oxidase (FPOX)
Fa'amatalaga
Ole enzyme e aoga mo le fuafuaina ole fructosyl-peptide ma le fructosyl-L-amino acid.
Fa'atonuga vaila'au
Mataupu Faavae o Tali
Fructosyl-peptide + H2O + O2→ Peptide + Glucosone + H2O2
Fa'amatalaga
| Mea Su'ega | Fa'amatalaga |
| Fa'amatalaga | Pa'u pa'epa'e amorphous, lyophilized |
| Gaoioiga | ≥4U/mg |
| Mama (SDS-PAGE) | ≥90% |
| Catalase | ≤0.01% |
| ATPase | ≤0.005% |
| Glucose oxidase | ≤0.03% |
| Cholesterol oxidase | ≤0.003% |
Felauaiga ma le teuina
Felauaiga: Ambient
Teuina:Teu i le -20°C(Taimi umi), 2-8°C (pu'upu'u)
Fautuaina le toe su'egaSoifua:2 tausaga
Tala'aga o atina'e
O se tasi o fa'ailoga o lo'o fa'aaogaina i su'esu'ega o le ma'isuka o le hemoglobin glycated (HbA1c).Ole fua ole HbA1c ile fa'aogaina ole enzymes e talafeagai mo le fa'agaioiina o le tele o fa'ata'ita'iga, ma e taugofie.E pei o lea, ua leva ona valaau malosi mai fomaʻi mo le atinaʻeina o sea suʻega enzyme.O le mea lea, na matou fausia ai se suʻega fou e faʻaaoga ai le "dipeptide method".Aemaise lava, na matou mauaina "Fructosyl-peptide Oxidase" (FPOX) lea e mafai ona faʻaaogaina e fai ma enzyme mo lenei suʻega.O lenei mea na faafaigofieina ai lo matou manuia i le ausiaina o se lalolagi muamua e ala i le faia o se mea moni o le HbA1c enzyme assay.O le "dipeptide method" lea e fa'aaoga ai le Protease (Proteolytic enzyme) e talepe ai le HbA1c i totonu o le toto, ona fua lea o le maualuga o dipeptides saccharified na gaosia i le FPOX.O lenei metotia na faʻafeiloaʻi ma le faʻafeiloaʻiga sili ona lelei ona o lona aoga o le faigofie, taugofie ma le saoasaoa, ma o le HbA1c fua faʻatatau e faʻaaoga ai le FPOX ua faʻaaogaina i le lalolagi atoa.
