Ribonuclease III (RNase III)

EC 3.1.24

Mama.: 2U/μL

afifi: 50μL,125μL

Fa'amatalaga Oloa

Fa'amatalaga

O lenei oloa o le ribonuclease III (RNase III) recombinantly faʻaalia e E. coli.O lenei exonuclease faʻapitoa e faʻapipiʻiina le RNA faʻalua (dsRNA) ma faʻatupuina 12-35bp dsRNA vaega faʻatasi ma le 5'-PO4 ma le 3'-OH, 3' overhangs

Fa'atonuga vaila'au

Fa'amatalaga Vaega

Fa'amatalaga o le iunite o gaoioiga: E tasi le iunite e fa'atatau i le aofa'i o le enzyme e mana'omia e fa'aleagaina ai le 1 μg o
dsRNA ile siRNA ile 50 μL reaction system ile 37°C mo le 20 minute.

Fa'amatalaga

Mea Su'ega	Fa'amatalaga
Gaoioiga Exonuclease	Fa'atuina < 0.1% o le aofa'i o le leitio
Gaoioiga Dnase Le'o Fa'apitoa	Le iloa
Su'esu'ega Fa'amama Polotini(SDS-PAGE)	≥ 95%
Gaoioiga RNase	＞ 90% ole RNA ole mea'ai e tumau pea