Peroxidase (punavai Horseradish) Synonym: Hydrogen peroxide oxidoreductase;HRP

Horseradish peroxidase (HRP) e vavae ese mai aʻa o le horseradish (Amoracia rusticana) ma e aofia i le ferroprotoporphyrin group of peroxidases.E faigofie ona tu'ufa'atasia le HRP ma le hydrogen peroxide (H2O2).Ole lavelave [HRP-H2O2] e mafai ona fa'ama'iina ai le tele o ituaiga o foa'i hydrogen:
Foa'i + H2O2 → Fo'ai Fa'asa'o + 2 H2O
Ole HRP ole a fa'ama'iina mea'ese'ese (silasila i le Laulau 1):
• Chromogenic
• Chemiluminescent (pei o le luminol po'o le isoluminol)
• Fluorogenic (e pei o le tyramine, homovanillic acid, poʻo le 4-hydroxyphenyl acetic acid)
O le HRP o se polypeptide filifili tasi e aofia ai alalaupapa disulfide e fa.HRP o se glycoprotein e aofia ai le 18% carbohydrate.O le gaʻo gaʻo e aofia ai galactose, arabinose, xylose, fucose, mannose, mannosamine, ma galactosamine, faʻalagolago i le isozyme patino.

O le HRP ose igoa fa'aoga lautele mo immunoglobulins i le tele o fa'aoga fa'ama'i fa'ama'i, e aofia ai immunoblotting, immunohistochemistry, ma ELISA.HRP e mafai ona faʻapipiʻiina i faʻamaʻi e ala i ni auala eseese, e aofia ai le glutaraldehyde, faʻamaʻi taimi, e ala i faʻamaufaʻailoga disulfide, faʻapea foʻi ma amino ma thiol faʻatonuga fesoʻotaʻiga.O le HRP o le faʻailoga sili ona manaʻomia mo faʻamaʻi, talu ai o le laʻititi laʻititi ma sili ona mautu o igoa e tolu e sili ona lauiloa o le enzyme (peroxidase, β-galactosidase, alkaline phosphatase) ma o lona glycosylation e taʻitaʻia ai le faʻaitiitia o le faʻaogaina o mea e le faʻapitoa.O se iloiloga o le glutaraldehyde ma le periodate conjugation auala ua lomia faasalalau.

O lo'o fa'aaogaina fo'i le peroxidase mo le su'eina o le kulukose4 ma le peroxyide i le vaifofo.O nisi o lomiga, 6-24 theses, 25-29 ma fa'asalalauga 30-46 ua ta'ua ai le fa'aogaina o le P8375 ia latou su'esu'ega.