Tasi Laasaga RT-qPCR pusa su'esu'e
Fa'amatalaga
U+ One Step RT-qPCR Probe Kit (Glycerol-free) o se glycerol-free one-step RT-qPCR reagent e faʻaaoga ai le RNA e fai ma faʻataʻitaʻiga (pei o le RNA virus), lea e talafeagai mo le atinaʻeina ma le mamanu o oloa lyophilized.O lenei oloa o loʻo tuʻufaʻatasia le faʻatinoga sili atu o le tasi-laasaga tuuto Reverse Transcriptase ma le vevela Champagne Taq DNA Polymerase, faʻatasi ai ma faʻamago vailaʻau faʻamago faʻamago lelei, lea e sili atu le faʻamalosia lelei, paleni ma faʻapitoa, ma e fetaui ma faʻamago faʻamago eseese. faʻagasologa. E le gata i lea, o le dUTP / UDG anti-pollution system o loʻo faʻaofiina i totonu o le reagent, lea e mafai ona galue i le vevela o le potu, faʻaumatia le aʻafiaga o le faʻaleagaina o oloa i luga o le qPCR, ma faʻamautinoa le saʻo o taunuuga.
Mataupu Faavae Autu o le RT-qPCR
Fa'amatalaga
| Su'ega Mea | Fa'amatalaga | I'uga |
| (SDS PAGE) Mama o le Enzyme Stock(SDS PAGE) | ≥95% | Pasia |
| Gaoioiga Endonuclease | Le iloa | Pasia |
| Gaoioiga Exodulease | Le iloa | Pasia |
| Gaoioiga Rnase | Le iloa | Pasia |
| E.coli DNA totoe | <1 kopi/60 | Pasia |
| Fuafuaga Su'ega-Faiga | 90%≤110% | Pasia |
Vaega
| Vaega | 100rxns | 1,000rns | 5,000 rxns |
| RNase leai se ddH2O | 2*1ml | 20ml | 100ml |
| 5 * tasi laʻasaga faʻafefiloi | 600μl | 6*1ml | 30ml |
| Tasi la'asaga fa'afefiloi enzyme | 150μl | 2*750μl | 7.5ml |
| 50* ROX fa'amatalaga Vai 1 | 60μl | 600μl | 3*1ml |
| 50* ROX fa'amatalaga Vai 2 | 60μl | 600μl | 3*1ml |
a.Tasi-Step Buffer e aofia ai le dNTP Mix ma le Mg2+.
e.O le Enzyme Mix e tele lava ina iai le fa'afeagai
transcriptase, Hot Start Taq DNA polymerase (suiga antibody) ma le inhibitor RNase.
i.Fa'aaogaina e fa'asa'o ai le sese o sinals fluorescene i le va o vaieli eseese.
i.ROX: E te manaʻomia le filifilia o le faʻavasegaina e tusa ai ma le faʻataʻitaʻiga o le meafaigaluega suʻega.
Talosaga
Su'esu'ega QPCR
Va'a ma Teuina
Felauaiga:pusa aisa
Tulaga Teuina:Teu i le -20℃.
Soifua Shief:18 masina
